Peer-Reviewed Journal Details
Mandatory Fields
Singh, I;Freeman, C;Heaney, F
2011
November
European Journal of Organic Chemistry
Efficient Synthesis of DNA Conjugates by Strain-Promoted Azide-Cyclooctyne Cycloaddition in the Solid Phase
Published
22 ()
Optional Fields
FREE CLICK CHEMISTRY 1,3-DIPOLAR CYCLOADDITION COPPER FUNCTIONALIZATION ACETYLENES PROTEIN
6739
6746
Conjugation of ligands to DNA oligonucleotides has been achieved in the solid phase by strain-promoted azide-alkyne cycloaddition (SPAAC). The oligonucleotide, modified with a simple nonfluroinated, monocyclic octyne, efficiently forms conjugates with a range of azide dipoles with varying steric and electronic characteristics. The reaction is clean and easily executed in a copper free environment at room temperature. It provides a variety of triazole-linked nucleic acid conjugates and is potentially useful in biotechnology and cell biology.
MALDEN
1434-193X
10.1002/ejoc.201101045
Grant Details