Peer-Reviewed Journal Details
Mandatory Fields
Collins, C;Keane, TM;Turner, DJ;O'Keeffe, G;Fitzpatrick, DA;Doyle, S
2013
June
Journal of Proteome Research
Genomic and Proteomic Dissection of the Ubiquitous Plant Pathogen, Armillaria mellea: Toward a New Infection Model System
Published
52 ()
Optional Fields
BASIDIOMYCETE PHANEROCHAETE-CHRYSOSPORIUM DE-N-ACETYLASES LACCARIA-BICOLOR CANDIDA-ALBICANS EXTRACELLULAR PROTEINS SUBSTRATE-SPECIFICITY PHYLOGENETIC ANALYSIS SPECIES PHYLOGENIES GENE SEQUENCE
12
2552
2570
Armillaria mellea is a major plant pathogen. Yet, no large-scale "-omics" data are available to enable new studies, and limited experimental models are available to investigate basidiomycete pathogenicity. Here we reveal that the A. mellea genome comprises 58.35 Mb, contains 14473 gene models, of average length 1575 bp (4.72 introns/gene). Tandem mass spectrometry identified 921 mycelial (n = 629 unique) and secreted (n = 183 unique) proteins. Almost 100 mycelial proteins were either species-specific or previously unidentified at the protein level. A number of proteins (n = 111) was detected in both mycelia and culture supernatant extracts. Signal sequence occurrence was 4-fold greater for secreted (50.2%) compared to mycelial (12%) proteins. Analyses revealed a rich reservoir of carbohydrate degrading enzymes, laccases, and lignin peroxidases in the A. mellea proteome, reminiscent of both basidiomycete and ascomycete glycodegradative arsenals. We discovered that A. mellea exhibits a specific killing effect against Candida albicans during coculture. Proteomic investigation of this interaction revealed the unique expression of defensive and potentially offensive A. mellea proteins (n = 30). Overall, our data reveal new insights into the origin of basidiomycete virulence and we present a new model system for further studies aimed at deciphering fungal pathogenic mechanisms.
WASHINGTON
1535-3893
10.1021/pr301131t
Grant Details