Peer-Reviewed Journal Details
Mandatory Fields
Kelly, J;Kavanagh, K
2010
June
Medical Mycology
Proteomic analysis of proteins released from growth-arrested Candida albicans following exposure to caspofungin
Published
11 ()
Optional Fields
CELL-WALL PROTEOME GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE ALCOHOL-DEHYDROGENASE IMMUNOGENIC PROTEINS GEL-ELECTROPHORESIS HUMAN-SERA IDENTIFICATION ANTIGENS ASPERGILLUS ANTIBODIES
48
598
605
The echinocandins (e. g., caspofungin) are a relatively new class of antifungal drugs that function by inhibiting the synthesis of beta-1,3-glucan in the cell wall and thus lead to lysis of the cell. In this work the effect of caspofungin on the release of peptides from non-growing cells of the yeast Candida albicans that had been exposed to the drug was monitored. Exposure to 0.19 mu g/ml caspofungin resulted in the release of amino acids from cells and of both small and large molecular weight proteins as demonstrated by 1- and 2-dimensional gel electrophoresis. Matrix-assisted laser desorption/ionizationtime of flight-mass spectrometry (MALDI-ToF) mass spectrometry was employed to identify a number of escaped peptides that were found to have increased in intensity upon exposure to the drug. A number of wall-associated proteins (e. g., phosphoglycerate kinase) and a number of glycolytic enzymes (phosphoglycerate mutase 1, fructose-bisphosphate aldolase) were identified. Importantly, several released proteins (e. g., pyruvate kinase, enolase 1, phosphoglycerate mutase, glyceraldehydes 3-phosphate dehydrogenase, fructose bisphosphate aldolase and alcohol dehydrogenase 1) are highly immunogenic in nature. The results presented here demonstrate that non-growing C. albicans cells are susceptible to the effect of caspofungin and that the caspofungin-mediated release of proteins from such cells could lead to a stronger immune response in vivo. This report illustrates that, in addition to hampering cell wall synthesis, caspofungin may also interfere with the permeability of the fungal cell wall.
ABINGDON
1369-3786
10.3109/13693780903405782
Grant Details