Peer-Reviewed Journal Details
Mandatory Fields
Fitzpatrick, DA;McInerney, JO
2005
February
Journal of Molecular Evolution
Evidence of positive Darwinian selection in Omp85, a highly conserved bacterial outer membrane protein essential for cell viability
Published
16 ()
Optional Fields
AMINO-ACID SITES NEISSERIA-MENINGITIDIS ESCHERICHIA-COLI PHYLOGENETIC ANALYSIS MAXIMUM-LIKELIHOOD EVOLUTION RECOMBINATION SUBSTITUTION GENE BIOGENESIS
60
268
273
Omp85 is a highly conserved outer membrane protein found in all gram-negative bacteria. It is essential for bacterial cell viability and plays an integral function in the positioning and folding of other outer membrane proteins into the bacterial outer membrane. We have employed a maximum likelihood and a maximum parsimony approach to detect evidence of positive Darwinian selection in Omp85 homologues from 10 delta-proteobacteria and have identified 14 amino acid sites that show evidence of being under the influence of adaptive evolution. Interestingly all sites bar one are concentrated within surface loops of the protein that most likely interact with host immune response or the surrounding environment. Alternatively amino acids within membrane-spanning regions of the protein are found to be under purifying selection most likely as a result of structural constraints.
NEW YORK
0022-2844
10.1007/s00239-004-0194-5
Grant Details