Peer-Reviewed Journal Details
Mandatory Fields
Bergin, D;Murphy, L;Keenan, J;Clynes, M;Kavanagh, K
2006
July
Microbes and Infection
Pre-exposure to yeast protects larvae of Galleria mellonella from a subsequent lethal infection by Candida albicans and is mediated by the increased expression of antimicrobial peptides
Published
82 ()
Optional Fields
DROSOPHILA-MELANOGASTER IMMUNE-RESPONSE WAX MOTH INSECTS CLONING INNATE HEMOLYMPH PROTEINS INVERTEBRATE VIRULENCE
8
2105
2112
Pre-exposure of the larvae of Galleria mellonella to Candida albicans or Saccharomyces cerevisiae protects against a subsequent infection with 10(6) C. albicans cells. This protection can also be induced by exposing larvae to glucan or laminarin prior to the administration of the potentially lethal inoculum. Analysis of the genes coding for galiomicin, a defensin in G. mellonella, a cysteine-rich antifungal peptide gallerimycin, an iron-binding protein transferrin and an inducible metalloproteinase inhibitor (IMPI) from G. mellonella demonstrated increased expression, which is at its highest after 24 h of the initial inoculum. Examination of the expression of proteins in the insect haemolymph using 2D electrophoresis and MALDI TOF analysis revealed an increased expression of a number of proteins associated with the insect immune response to infection 24 h after the initial exposure. This study demonstrates that the larvae of G. mellonella can withstand a lethal inoculum of C. albicans if pre-exposed to a non-lethal dose of yeast or polysaccharide 24 h previously which is mediated by increased expression of a number of antimicrobial peptides and the appearance of a number of peptides in the challenged larvae. (c) 2006 Elsevier SAS. All rights reserved.
AMSTERDAM
1286-4579
10.1016/j.micinf.2006.03.005
Grant Details