Book Chapter Details
Mandatory Fields
O'Shaughnessy, L;Doyle, S
2011 January
PROTEIN CHROMATOGRAPHY: METHODS AND PROTOCOLS
Purification of Proteins from Baculovirus-Infected Insect Cells
HUMANA PRESS INC
TOTOWA
Published
1
Optional Fields
ASPERGILLUS-FUMIGATUS RECOMBINANT PROTEINS EXPRESSION TAG
Expression of recombinant proteins in the baculovirus/insect cell expression system is employed because it enables post-translational protein modification and high yields of recombinant protein. The system is capable of facilitating the functional expression of many proteins - either secreted or intracellularly located within infected insect cells. Strategies for the isolation and extraction of soluble proteins are presented in this chapter and involve selective cell lysis, precipitation and chromatography. Protein insolubility, following recombinant expression in insect cells, can occur. However, using the methods described herein, it is possible to extract and purify insoluble protein using affinity, ion-exchange and gel filtration chromatography. Indeed, protein insolubility often aids protein purification.
1064-3745
295
309
10.1007/978-1-60761-913-0_16
Grant Details