Peer-Reviewed Journal Details
Mandatory Fields
Marques, MPC;Walshe, K;Doyle, S;Fernandes, P;de Carvalho, CCCR
2012
March
Process Biochemistry
Anchoring high-throughput screening methods to scale-up bioproduction of siderophores
Published
3 ()
Optional Fields
FATTY-ACID ANALYSIS ESCHERICHIA-COLI PROTEIN EXPRESSION CHELATING-AGENTS DISSOLVED-OXYGEN BIOREACTORS BACTERIA QUANTIFICATION IDENTIFICATION FERMENTATIONS
47
416
421
The use of high throughput strategies is of acknowledged relevance since the rational use of small-scale reactors, coupled with suitable analytic tools, is contributing to the acceleration of process development in several areas of biotechnology. These small-scale reactors are available in different working volumes and configurations, being useful in a wide array of applications, from cell screening to process optimization. The present work was focused on the development of a high-throughput strategy, combining microtiter plates and analytic methodologies, to screen an in-house library of environmental bacteria in order to identify good siderophore producers. From a library of roughly 500 marine microorganisms, it was possible to ultimately obtain 11 bacterial strains with high production capabilities. Two of them had not been previously identified as siderophore producers. The bioprocess was scaled-up from microtiter plates to a 5 L stirred tank reactor, while maintaining the overall volumetric productivity, using the 10,a similarity as scale-up criterion. This novel approach is a suitable alternative to traditional screening tools. (C) 2011 Elsevier Ltd. All rights reserved.
OXFORD
1359-5113
10.1016/j.procbio.2011.11.020
Grant Details