Peer-Reviewed Journal Details
Mandatory Fields
Cotter, G;Weedle, R;Kavanagh, K
1998
January
Mycopathologia
Monoclonal antibodies directed against extracellular matrix proteins reduce the adherence of Candida albicans to HEp-2 cells
Published
11 ()
Optional Fields
SURFACE HYDROPHOBICITY EPITHELIAL-CELLS BLOCK ADHERENCE ADHESION RECEPTOR
141
137
142
The presence of the extracellular matrix (ECM) proteins collagen types I and IV, laminin and fibronectin on the surface of HEp-2 cells was confirmed by flow cytometry using monoclonal antibodies. Monoclonal antibodies directed against these ECM proteins reduced the adherence of C. albicans ATCC 44990 to KEp-2 cells, the greatest reductions being evident in assays which incorporated anti-collagen type IV monoclonal antibody. The ability of sugaramines to inhibit the adherence of C. albicans; to a variety of cell types has been demonstrated previously and the most significant reduction in C. albicans - HEp-2 adherence was in assays which incorporated 0.2 M galactosamine. The combination of anti-collagen IV monoclonal antibody and galactosamine reduced the adherence of C albicans to HEp-2 cells by approximately 70% (p < 0.05).
DORDRECHT
0301-486X
Grant Details