Peer-Reviewed Journal Details
Mandatory Fields
Fallon, JP;Troy, N;Kavanagh, K
2011
September
Virulence
Pre-exposure of Galleria mellonella larvae to different doses of Aspergillus fumigatus conidia causes differential activation of cellular and humoral immune responses
Published
46 ()
Optional Fields
CANDIDA-ALBICANS INSECT IMMUNITY PROTEINS HEMOCYTES INFECTION SYSTEM GLYCOPROTEIN RECOGNITION HEMOLYMPH VIRULENCE
2
413
421
Larvae of Galleria mellonella are useful models for studying the virulence of microbial pathogens or for evaluating the potency of antimicrobial agents. In this work we demonstrated that prior exposure of larvae to non-lethal doses of Aspergillus fumigatus conidia increases the resistance of larvae to a lethal dose (1 x 10(7) 20 mu l(-1)) 24 h later. Exposure of larvae to a conidia concentration of 1 x 10(4) 20 mu l(-1) leads to an increase in hemocyte density but an inoculum of 1 x 10(5) conidia leads to enhanced expression of antimicrobial peptides, increased binding of proteins (e.g., arylophorin, prophenoloxidase, apolipophorin) to conidia and elevated hemocyte density. These results suggest that a low dose of conidia (1 x 10(4)) predominantly activates the cellular immune response but that a higher dose (1 x 10(5)) that is still not lethal activates a humoral immune response to the greatest extent. While insects have an immune system analogous to the innate immune response of mammals these results suggest that it is capable of assessing the extent of the microbial challenge and mounting a 'proportionate' immune response which may have important survival advantages.
AUSTIN
2150-5594
10.4161/viru.2.5.17811
Grant Details