Peer-Reviewed Journal Details
Mandatory Fields
Doyle, SL;Shirey, KA;McGettrick, AF;Kenny, EF;Carpenter, S;Caffrey, BE;Gargan, S;Quinn, SR;Caamano, JH;Moynagh, P;Vogel, SN;O'Neill, LA
2013
August
Journal of Biological Chemistry
Nuclear Factor kappa B2 p52 Protein Has a Role in Antiviral Immunity through I kappa B Kinase epsilon-dependent Induction of Sp1 Protein and Interleukin 15
Published
11 ()
Optional Fields
TRANSCRIPTION FACTOR UP-REGULATION IKK-EPSILON GENE-EXPRESSION SPLENIC MICROARCHITECTURE SIGNALING PATHWAY IE2 PROTEIN IL-15 GENE ACTIVATION PROMOTER
288
25066
25075
In this study we describe a previously unreported function for NF kappa B2, an NF kappa B family transcription factor, in antiviral immunity. NF kappa B2 is induced in response to poly(I: C), a mimic of viral dsRNA. Poly(I: C), acting via TLR3, induces p52-dependent transactivation of a reporter gene in a manner that requires the kinase activity of I kappa B kinase epsilon (IKK epsilon) and the transactivating potential of RelA/p65. We identify a novel NF kappa B2 binding site in the promoter of the transcription factor Sp1 that is required for Sp1 gene transcription activated by poly(I: C). We show that Sp1 is required for IL-15 induction by both poly(I: C) and respiratory syncytial virus, a response that also requires NF kappa B2 and IKK epsilon. Our study identifies NF kappa B2 as a target for IKK epsilon in antiviral immunity and describes, for the first time, a role for NF kappa B2 in the regulation of gene expression in response to viral infection.
BETHESDA
0021-9258
10.1074/jbc.M113.469122
Grant Details