Peer-Reviewed Journal Details
Mandatory Fields
KAVANAGH, K;WALSH, M;WHITTAKER, PA
1991
July
Fems Microbiology Letters
ENHANCED INTRASPECIFIC PROTOPLAST FUSION IN YEAST
Published
6 ()
Optional Fields
POLYETHYLENE-GLYCOL MEMBRANE-FUSION CANDIDA-ALBICANS PLANT-PROTOPLASTS HYBRIDIZATION INDUCTION
81
283
286
Intraspecific hybrid production from the polyethylene glycol induced fusion of yeast protoplasts was greatly increased when calcium propionate was included as the source of the requisite Ca2+. The use of calcium propionate, as opposed to the more commonly employed calcium chloride, resulted in substantially greater yields of hybrids from intraspecific fusions of protoplasts of Saccharomyces cerevisiae and Candida albicans. It is postulated that the ability of calcium propionate to enhance the fusion frequency is due to the anion binding to the etheric oxygen of PEG and potentiating the fusogenicity of the polymer.
AMSTERDAM
0378-1097
Grant Details