Peer-Reviewed Journal Details
Mandatory Fields
English, K;Barry, FP;Mahon, BP
2008
January
Immunology Letters
Murine mesenchymal stem cells suppress dendritic cell migration, maturation and antigen presentation
Published
165 ()
Optional Fields
MARROW STROMAL CELLS INHIBIT LYMPHOCYTE-PROLIFERATION MOUSE BONE-MARROW INTERFERON-GAMMA BORDETELLA-PERTUSSIS LANGERHANS CELLS PRESENTING CELL E-CADHERIN T-CELLS IN-VIVO
115
50
58
Mesenchymal stem cells (MSC) possess a wide range of immunosuppressive functions. Among these is the ability to inhibit CD4(+) T cell proliferation. Dendritic cells (DC) play a role in initiating cell-mediated immunity; however, the immunosuppressive influence of MSC on professional antigen presenting cells remains unclear. DC exposed to TNF-alpha and cultured with murine MSC failed to show regular upregulation of maturation markers. Similarly, the presence of MSC abrogated the capacity of ovalbumin-pulsed DC to support antigen specific CD4(+) T cell proliferation, or for DC to display an MHC class II- peptide complex recognizable by specific antibody. Interestingly, culture of MSC with DC resulted in reduced expression of CCR7 by DC following stimulation. Likewise, DC matured in the presence of MSC, showed significantly less migration to CCL19. In contrast, murine MSC prevented loss of expression of the tissue anchoring protein E-cadherin by DC. Modulation of DC maturation and function was not permanent and could be restored after removal of MSC. These data demonstrate that MSC modulate the three cardinal features of DC maturation, providing the first demonstration of MSC interference with DC migration. (C) 2007 Elsevier B.V. All rights reserved.
AMSTERDAM
0165-2478
10.1016/j.imlet.2007.10.002
Grant Details