Peer-Reviewed Journal Details
Mandatory Fields
Daly, P;Collier, T;Doyle, S
2002
January
Letters in Applied Microbiology
PCR-ELISA detection of Escherichia coli in milk
Published
66 ()
Optional Fields
IN-GROUND BEEF RAPID DETECTION FOODS O157-H7 MEDIA ASSAY GENE DNA
34
222
226
Aims: The purpose of this study was to develop a reliable molecular procedure for the detection of Escherichia coli in milk. Methods and Results: Robust and expeditious DNA extraction and PCR techniques were evaluated using Enzyme-Linked Immunosorbent Assay (ELISA) detection of biotin-labelled amplicons to facilitate optimal detection of E. coli DNA. Conclusions: It was found that 5 E. coli colony-forming units (cfu) could be detected per PCR reaction using the PCR-ELISA system, equating to a sensitivity of detection of 100 E. coli cfu ml(-1) pasteurized milk. Significance and Impact of the Study: This approach should facilitate evaluation of milk contamination and enable rapid detection of E. coli mastitis, leading to correct deployment of relevant antibiotic therapy and improved animal welfare.
OXFORD
0266-8254
Grant Details