Peer-Reviewed Journal Details
Mandatory Fields
Geraghty, P;Kavanagh, K
2003
April
Archives of Microbiology
Disruption of mitochondrial function in Candida albicans leads to reduced cellular ergosterol levels and elevated growth in the presence of amphotericin B
Published
33 ()
Optional Fields
SACCHAROMYCES-CEREVISIAE PROTOPLAST FUSION PETITE MUTATION IN-VITRO ANTIFUNGAL SUSCEPTIBILITY DRUG-RESISTANCE YEAST GLABRATA FLUCONAZOLE INDUCTION
179
295
300
A respiratory-deficient mutant of Candida albicans MEN was generated by culturing cells in medium supplemented with ethidium bromide at 37 degreesC for 5 days. The respiratory-deficient mutant (C. albicans MMU11) was incapable of growth on glycerol, had a reduced oxygen uptake rate and demonstrated an altered mitochondrial cytochrome profile. Respiratory-competent cybrids were formed by mitochondrial transfer following fusion of protoplasts with those of C. albicans ATCC 44990. Mutant MMU11 possessed lower levels of ergosterol than the parental isolates and the cybrids, and demonstrated a small but statistically significant increase in tolerance to amphotericin B. The results demonstrated that disruption of mitochondrial function in C. albicans increases the tolerance to amphotericin B, possibly mediated by a reduction in cellular ergosterol content.
NEW YORK
0302-8933
10.1007/s00203-003-0530-y
Grant Details