Peer-Reviewed Journal Details
Mandatory Fields
Reiber, K;Reeves, EP;Neville, CM;Winkler, R;Gebhardt, P;Kavanagh, K;Doyle, S
2005
July
Fems Microbiology Letters
The expression of selected non-ribosornal peptide synthetases in Aspergillus fumigatus is controlled by the availability of free iron
Published
36 ()
Optional Fields
SIDEROPHORE BIOSYNTHESIS ANTIBIOTIC BIOSYNTHESIS ADENYLATION DOMAINS USTILAGO-MAYDIS GENE VIRULENCE PROTEINS IDENTIFICATION POLYKETIDE NIDULANS
248
83
91
Three non-ribosomal peptide synthetase genes, termed sidD, sidC and sidE, have been identified in Aspergillus fumigatus. Gene expression analysis by RT-PCR confirms that expression of both sidD and C was reduced by up to 90% under iron-replete conditions indicative of a likely role in siderophore biosynthesis. SUE expression was less sensitive to iron levels. In addition, two proteins purified from mycelia grown under iron-limiting conditions corresponded to SidD (similar to 200 kDa) and SidC (496 kDa) as determined by MALDI ToF peptide mass fingerprinting and MALDI LIFT-ToF/ToF. Siderophore synthetases are unique in bacteria and fungi and represent an attractive target for antimicrobial chemotherapy. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
AMSTERDAM
0378-1097
10.1016/j.femsle.2005.05.028
Grant Details