Peer-Reviewed Journal Details
Mandatory Fields
Corcoran, A;Mahon, BP;Doyle, S
2004
May
Journal of Infectious Diseases
B cell memory is directed toward conformational epitopes of parvovirus B19 capsid proteins and the unique region of VP1
Published
25 ()
Optional Fields
IMMUNE-RESPONSES BACULOVIRUS EXPRESSION PREGNANT-WOMEN INFECTION ANTIGEN VIRUS ASSAY IGG CHILDREN NS1
189
1873
1880
Background. Loss of antibody reactivity against linear epitopes of parvovirus B19 (B19) capsid proteins VP1 and VP2 occurs after infection; however, it is unclear whether B cell memory is established against linear epitopes. Methods. B cell enzyme-linked immunospot assay was used to evaluate B19-specific B cell memory in volunteer donors (n = 22). Results. B cell memory is maintained against conformational epitopes of VP2 and is absent against linear epitopes of VP2. Individuals seronegative for IgG against the unique region of VP1 have detectable B cell memory, with the potential to mount a humoral response on reexposure to B19. Conversely, in mice immunized with VP2, long-lasting IgG against linear epitopes of VP2 and a strong B cell - memory response are observed. Conclusions. B cell memory is established and maintained against conformational epitopes of VP2 and against linear epitopes of VP1 but not against linear epitopes of VP2. These findings further our understanding of the immune response to B19 and suggest that analysis of B19-specific B cell memory merits consideration for future B19-vaccine studies.
CHICAGO
0022-1899
Grant Details