Peer-Reviewed Journal Details
Mandatory Fields
Reiber K.;Reeves E.;Neville C.;Winkler R.;Gebhardt P.;Kavanagh K.;Doyle S.
2005
July
Fems Microbiology Letters
The expression of selected non-ribosomal peptide synthetases in Aspergillus fumigatus is controlled by the availability of free iron
Published
()
Optional Fields
4′-PPTase Iron MALDI ToF NRPS Proteomics Secondary metabolites Siderophore
248
1
83
91
Three non-ribosomal peptide synthetase genes, termed sidD, sidC and sidE, have been identified in Aspergillus fumigatus. Gene expression analysis by RT-PCR confirms that expression of both sidD and C was reduced by up to 90% under iron-replete conditions indicative of a likely role in siderophore biosynthesis. SidE expression was less sensitive to iron levels. In addition, two proteins purified from mycelia grown under iron-limiting conditions corresponded to SidD (∼200 kDa) and SidC (496 kDa) as determined by MALDI ToF peptide mass fingerprinting and MALDI LIFT-ToF/ToF. Siderophore synthetases are unique in bacteria and fungi and represent an attractive target for antimicrobial chemotherapy. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
0378-1097
10.1016/j.femsle.2005.05.028
Grant Details