Peer-Reviewed Journal Details
Mandatory Fields
Ao, X;Bright, SA;Taylor, NC;Elmes, RBP
2017
August
Organic and Biomolecular Chemistry
2-Nitroimidazole based fluorescent probes for nitroreductase; monitoring reductive stress in cellulo
Published
34 ()
Optional Fields
OFF-ON PROBE TUMOR HYPOXIA SELECTIVE DETECTION CANCER-THERAPY IMAGING AGENTS ANTICANCER DISEASE TH-302
15
6104
6108
Two 2-nitroimidazole-1,8-naphthalimide conjugates, 1 and 2, have been synthesised as fluorescence probes for the detection of reductive stress in HeLa cells. The 4-substituted derivative 1 was shown to act as a highly sensitive and selective substrate for nitroreductase where it exhibited a clear blue to green ratiometric fluorescence response visible to the naked eye. Moreover, biological studies demonstrated 1 could be activated in cellulo where the impact of reductive stress was easily monitored using confocal microscopy and flow cytommetry.
CAMBRIDGE
1477-0520
10.1039/c7ob01406f
Grant Details