Peer-Reviewed Journal Details
Mandatory Fields
Masterson JC;Biette KA;Hammer JA;Nguyen N;Capocelli KE;Saeedi BJ;Harris RF;Fernando SD;Hosford LB;Kelly CJ;Campbell EL;Ehrentraut SF;Ahmed FN;Nakagawa H;Lee JJ;McNamee EN;Glover LE;Colgan SP;Furuta GT;
2019
July
Journal of Clinical Investigation
Epithelial HIF-1α/claudin-1 axis regulates barrier dysfunction in eosinophilic esophagitis.
Published
12 ()
Optional Fields
129
8
Epithelial barrier dysfunction is a significant factor in many allergic diseases, including eosinophilic esophagitis (EoE). Infiltrating leukocytes and tissue adaptations increase metabolic demands and decrease oxygen availability at barrier surfaces. Understanding of how these processes impact barrier is limited, particularly in allergy. Here, we identified a regulatory axis whereby the oxygen-sensing transcription factor HIF-1α orchestrated epithelial barrier integrity, selectively controlling tight junction CLDN1 (claudin-1). Prolonged experimental hypoxia or HIF1A knockdown suppressed HIF-1α-dependent claudin-1 expression and epithelial barrier function, as documented in 3D organotypic epithelial cultures. L2-IL5OXA mice with EoE-relevant allergic inflammation displayed localized eosinophil oxygen metabolism, tissue hypoxia, and impaired claudin-1 barrier via repression of HIF-1α/claudin-1 signaling, which was restored by transgenic expression of esophageal epithelial-targeted stabilized HIF-1α. EoE patient biopsy analysis identified a repressed HIF-1α/claudin-1 axis, which was restored via pharmacologic HIF-1α stabilization ex vivo. Collectively, these studies reveal HIF-1α's critical role in maintaining barrier and highlight the HIF-1α/claudin-1 axis as a potential therapeutic target for EoE.
1558-8238
10.1172/JCI126744
Grant Details