Peer-Reviewed Journal Details
Mandatory Fields
Stockmann, H;O'Flaherty, R;Adamczyk, B;Saldova, R;Rudd, PM
2015
January
Integrative Biology
Automated, high-throughput serum glycoprofiling platform
Published
33 ()
Optional Fields
N-GLYCANS GLYCOSYLATION GLYCOMICS GLYCOPROTEINS DISEASE
7
1026
1032
Complex carbohydrates are rapidly becoming excellent biomarker candidates because of their high sensitivity to pathological changes. However, the discovery of clinical glycobiomarkers has been slow, due to the scarcity of high-throughput glycoanalytical workflows that allow rapid glycoprofiling of large clinical sample sets. To generate high-quality quantitative glycomics data in a high-throughput fashion, we have developed a robotized platform for rapid serum-based N-glycan sample preparation. The sample preparation workflow features a fully automated, rapid glycoprotein denaturation followed by sequential enzymatic glycan release, glycan purification on solid-supported hydrazide and fluorescent labelling. This allows accurate glycan quantitation by ultra-high performance liquid chromatography (UPLC). The sample preparation workflow was automated using an eight-channel Hamilton Robotics liquid handling workstation, allowing the preparation of almost 100 samples in 14 hours with excellent reproducibility and thus should greatly facilitate serum-based glyco-biomarker discovery.
CAMBRIDGE
1757-9694
10.1039/c5ib00130g
Grant Details